精品三级国产精品经典三-精品三级国产一区二区三区四区-精品三级久久久久久久电影-精品三级三级三级三级三级-正在播放一区-正在播放亚洲一区

產(chǎn)品中心您現(xiàn)在的位置:首頁 > 產(chǎn)品展示 > 化學(xué)試劑 > Kerafast > EQ0001Kerafast抗體Anti-Puromycin 3RH11

Kerafast抗體Anti-Puromycin 3RH11

更新時(shí)間:2025-02-26

簡(jiǎn)要描述:

Kerafast抗體Anti-Puromycin [3RH11]:measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo.

Kerafast抗體Anti-Puromycin [3RH11]

 

背景介紹:

貨號(hào):EQ0001,嘌呤霉素單克隆抗體提供了一種非放射性方法來測(cè)量與嘌呤霉素孵育的細(xì)胞或組織切片中的整體蛋白質(zhì)合成(mRNA 翻譯)速率,或體內(nèi)用嘌呤霉素處理的動(dòng)物。

特色:

* 允許直接使用標(biāo)準(zhǔn)免疫化學(xué)方法對(duì)翻譯進(jìn)行簡(jiǎn)單的評(píng)估和量化

* 傳統(tǒng)脈沖追蹤方法的有利替代方法,后者依賴于放射性氨基酸標(biāo)記

* 與蛋白質(zhì)印跡和 ELISA 應(yīng)用兼容

* 使用 Absolute Antibody 的重組平臺(tái)制造,具有來自雜交瘤 3RH11 的可變區(qū)(即特異性)

 

嘌呤霉素是一種氨基核苷類抗生素,來源于白化鏈霉菌,在核糖體中發(fā)生翻譯過程中導(dǎo)致鏈過早終止。該分子的一部分類似于氨酰化 tRNA 的 3' 端,使其可用于蛋白質(zhì)翻譯分析。用于監(jiān)測(cè)蛋白質(zhì)合成的經(jīng)典脈沖追蹤或淹沒劑量方法依賴于放射性蛋氨酸和(半)氨酸標(biāo)記的測(cè)量。使用嘌呤霉素免疫檢測(cè)進(jìn)行分析是放射性氨基酸標(biāo)記的一種有利替代方法,并且允許使用標(biāo)準(zhǔn)免疫化學(xué)方法直接評(píng)估/量化翻譯

 

EQ0001,This monoclonal antibody to puromycin provides a non-radioactive method to measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo.

Highlights:

* Allows for the simple evaluation and quantification of translation directly using standard immunochemical methods

* Advantageous alternative to traditional pulse-chase methods, which rely on radioactive amino acid labeling

* Compatible with Western Blot and ELISA applications

* Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 3RH11

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine and cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, and allows for the evaluation/quantification of translation directly using standard immunochemical methods


 

Product Type: Antibody
Name: Anti-Puromycin (3RH11)
Host: Mouse
Isotype: IgG1 kappa
Clonality: Monoclonal
Clone Name: 3RH11
Specificity: This antibody recognizes puromycin.
Immunogen: puromycin hydrochloride
Format: Liquid
Purification Method: Protein G purified
Buffer: PBS with 0.02% Proclin 300
Tested Applications: Western blotting (1:1,000), ELISA and Immunofluorescence microscopy.
Storage: +4C (short-term), -20C (long-term); Avoid repeated freeze/thaw cycles.
Shipped: Cold packs

 

數(shù)據(jù)展示:

 

(A) C2C12 myoblasts were starved of serum and leucine for 2 hr and then IGF-1 and leucine were added to the medium of some of the cells for 45 min. Puromycin (1uM) was added to the medium of some of the cells (lanes 3-6) 30 min before harvest. (B) Quantification of western blot analysis from panel A. (C) In the same study, but in a separate set of culture dishes, cells were incubated with [35S]methionine instead of puromycin and incorporation was measured.

 

 

A Western blot was run using the same samples where one set was run on the left side of the gel and the other on the right.  The left side was probed with our original monoclonal anti-puromycin antibody and the other side was probed with the recombinant anti-puromycin antibody, both at 1:1,000 dilution. The secondary antibody was used at the same dilution for both sides and they were both exposed for ~40 sec. The first two samples were from skeletal muscle of mice where the first lane is muscle from the control hindlimb and the other is from a hindlimb that had been immobilized with a cast for three days.  The other 3 lanes are from HEK393T cells: the first lane is from cells incubated in complete medium, the middle lane is from cells incubated for 2 hr in medium lacking glucose and serum, and the last lane is cells incubated for 1.5 hr without glucose or serum and then glucose and serum were returned during the last 30 min.  As expected, puromycin incorporation was lower in the immobilized hindlimb compared to the contralateral control hindlimb, and also in cells deprived of glucose and serum compared to cells in complete medium.  Resupplementation partially restored incorporation.

 

Kerafast抗體Anti-Puromycin [3RH11]已發(fā)表文獻(xiàn)

參考文獻(xiàn):

1. Lee KH, Zhang P, Kim HJ, Mitrea DM, Sarkar M, Freibaum BD, Cika J, Coughlin M, Messing J, Molliex A, Maxwell BA, Kim NC, Temirov J, Moore J, Kolaitis RM, Shaw TI, Bai B, Peng J, Kriwacki RW, Taylor JP. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell. 2016 Oct 20;167(3):774-788.e17.

2. Tom Dieck S, Kochen L, Hanus C, Heumüller M, Bartnik I, Nassim-Assir B, Merk K, Mosler T, Garg S, Bunse S, Tirrell DA, Schuman EM. Direct visualization of newly synthesized target proteins in situ. Nat Methods. 2015 May;12(5):411-4.

3. Gantois I, Khoutorsky A, Popic J, Aguilar-Valles A, Freemantle E, Cao R, Sharma V, Pooters T, Nagpal A, Skalecka A, Truong VT, Wiebe S, Groves IA, Jafarnejad SM, Chapat C, McCullagh EA, Gamache K, Nader K, Lacaille JC, Gkogkas CG, Sonenberg N. Metformin ameliorates core deficits in a mouse model of fragile X syndrome. Nat Med. 2017 Jun;23(6):674-677.

4. Rangaraju V, Lauterbach M, Schuman EM. Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity. Cell. 2019 Jan 10;176(1-2):73-84.e15.

5. Hafner AS, Donlin-Asp PG, Leitch B, Herzog E, Schuman EM. Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments. Science. 2019 May 17;364(6441).

6. Hörnberg H, Pérez-Garci E, Schreiner D, et al. Rescue of oxytocin response and social behaviour in a mouse model of autism. Nature. 2020;584(7820):252-256.

 

靶點(diǎn)科技(北京)有限公司,現(xiàn)貨庫存,隨時(shí)發(fā)貨。訂購前建議技術(shù)咨詢,我們的技術(shù)團(tuán)隊(duì)可以提供免費(fèi)技術(shù)方案咨詢。

 


 

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
靶點(diǎn)科技(北京)有限公司

靶點(diǎn)科技(北京)有限公司

地址:中關(guān)村生命科學(xué)園北清創(chuàng)意園2-4樓2層

© 2025 版權(quán)所有:靶點(diǎn)科技(北京)有限公司  備案號(hào):京ICP備18027329號(hào)-2  總訪問量:294935  站點(diǎn)地圖  技術(shù)支持:化工儀器網(wǎng)  管理登陸

主站蜘蛛池模板: 亚洲免费精品视频 | 波多野结衣在线观看免费区 | 国产精品女在线观看 | 欧美成人午夜毛片免费影院 | 成年人免费视频观看 | 国产精品日本欧美一区二区 | 久久99精品久久久久久青青91 | 美女扒开腿让男人桶 | 免费欧美一级 | 国产午夜精品久久理论片 | 青青热久久综合网伊人 | 国产一区二区久久 | 自拍视频啪 | 欧美一区三区 | 免费公开视频人人人人人人人 | 一区二区三区免费视频观看 | 国产激情一区二区三区 | 亚洲国产天堂久久综合网站 | 亚洲欧美自拍偷拍 | 免费观看呢日本天堂视频 | 欧美精品片| 成年男人的天堂 | 国产三级a三级三级午夜 | 免费看欧美日韩一区二区三区 | 美国毛片毛片全部免费 | 高清免费国产在线观看 | 国产精品视频免费 | 国产精品福利午夜一级毛片 | 久久久999国产精品 久久久99精品免费观看 | 国产精品久久久久久一级毛片 | 未成人做爰视频www 窝窝午夜精品一区二区 | 国产成人禁片免费观看 | 欧美一级视频免费看 | 免费一级毛片在线观看 | 400部大量精品情侣网站 | 亚洲国产精品影院 | 亚洲精品男人天堂 | 国模肉肉人体大尺度啪啪 | 国内精品久久久久久网站 | 欧美99视频| 91久久亚洲精品国产一区二区 |