生物素-PEG3-疊氮化物是一種不可裂解的疊氮化物活化生物素化試劑，通過銅催化的點(diǎn)擊反應(yīng)與末端炔烴反應(yīng)反應(yīng)，通過無銅點(diǎn)擊反應(yīng)與應(yīng)變的環(huán)辛炔（例如DBCO或BCN化合物）反應(yīng)，或通過稱為Staudinger化學(xué)的機(jī)制與磷化氫標(biāo)記的分子反應(yīng)，從而實現(xiàn)生物樣品中衍生化分子的高效和特異性偶聯(lián)。這些反應(yīng)是生物正交的，或者換句話說，與生物系統(tǒng)相容，因為它的成分不與生物環(huán)境發(fā)生反應(yīng)。

Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. These reactions are bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.